The hunky trio were rivalled by The Vampire Diaries star Ian Somerhalder, who was voted third in the survey, proving that women can't get enough of men with fangs. Glamour's acting editor Charlotte Duck said: "Women are attracted to vampires because of the element of danger that comes with them. They are the ultimate bad boys -- and women want to tame the beasts."
The list -- which was compiled from over 50,000 votes by readers of Glamour.com -- also included many shocks, with many attractive stars being placed further down the list than in previous years.
Johnny Depp dropped from second place to sixth, while soccer star David Beckham slipped from fourth down to 21st.
Hollywood legend George Clooney only reached position 35 on the list, while Leonardo DiCaprio, Keanu Reeves and Jude Law -- who have scored highly in the survey in recent years -- did not even feature.
Glamour’s Top 20 Sexiest Men of 2010
1. Robert Pattinson
2. Taylor Lautner
3. Ian Somerhalder
4. Xavier Samuel
5. Kellan Lutz
6. Johnny Depp
7. Justin Bieber
8. Gerard Butler
9. Hayden Christensen
10. Channing Tatum
11. Orlando Bloom
12. Chace Crawford
13. Chris Brown
14. Justin Timberlake
15. Christiano Ronaldo
16. Ryan Gosling
17. Ed Westwick
18. Ashton Kutcher
19. Jake Gyllenhaal
20. Bradley Cooper